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Human Soluble CD4 Recombinant

CD4

accession:  P01730
   Size A     20ug   $ 160
   Size B      100ug  $480
   Size C      500ug  $ 1950
Domain  :  IG
Gene  :  CD4
Catalog no. :  RKP01730
Source:
Optimized DNA sequence encoding Human CD4 extracellular domain was expressed in HEK293 cells.

 
Molecular weight:
Human soluble CD4 is generated by the proteolytic removal of the extracellular domain
and propeptide, the molecule has a calculated molecular mass of approximately 40 kDa.

Recombinant CD4 is a disulfide-linked monomer protein consisting of 376 amino acid residue subunits, and migrates due to glycosylation as an approximately 46 kDa protein under non-reducing and reducing conditions in SDS-PAGE.


 
Purity:
>90%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The activity was tested by immobilized recombinant CD4 (1ug/ml) ability to support adhesion of HeLa cells (5x104 cells/well ) , 40-80% will adhere after 60min at 37C.

 
Protein Sequence:
        10         20         30         40         50         60 
MNRGVPFRHL LLVLQLALLP AATQGKKVVL GKKGDTVELT CTASQKKSIQ FHWKNSNQIK 

        70         80         90        100        110        120 
ILGNQGSFLT KGPSKLNDRA DSRRSLWDQG NFPLIIKNLK IEDSDTYICE VEDQKEEVQL 

       130        140        150        160        170        180 
LVFGLTANSD THLLQGQSLT LTLESPPGSS PSVQCRSPRG KNIQGGKTLS VSQLELQDSG 

       190        200        210        220        230        240 
TWTCTVLQNQ KKVEFKIDIV VLAFQKASSI VYKKEGEQVE FSFPLAFTVE KLTGSGELWW 

       250        260        270        280        290        300 
QAERASSSKS WITFDLKNKE VSVKRVTQDP KLQMGKKLPL HLTLPQALPQ YAGSGNLTLA 

       310        320        330        340        350        360 
LEAKTGKLHQ EVNLVVMRAT QLQKNLTCEV WGPTSPKLML SLKLENKEAK VSKREKAVWV 

       370        380        390        400        410        420 
LNPEAGMWQC LLSDSGQVLL ESNIKVLPTW STPVQPMALI VLGGVAGLLL FIGLGIFFCV 

       430        440        450        460 
RCRHRRRQAE RMSQIKRLLS EKKTCQCPHR FQKTCSPI
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant CD4 was lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

CD4

CD4 is a 55 kDa transmembrane glycoprotein belonging to the immunoglobulin superfamily. In humans CD4 is expressed of peripheral T-cells, thymocytes, on macrophages and granulocytes. CD4 acts as a cellular adhesion molecule binding MHC class 2 molecules. It stabilizes the interaction of MHC class 2 restricted T- cells and antigen-presenting cells expressing an antigen in combination with a MHC class 2 molecule. Interactions of CD4 with MHC class 2 molecules are crucial during thymic development and subsequently for the function of single- positive CD4(+), CD8(-) T- lymphocytes. CD4 functions as an effector molecule for intracellular signal transduction. In T- lymphocytes CD4 has been found to be associated with tyrosine kinase lck which phosphorylates a number of other cell surface proteins and is involved probably in transducing the intracellular signal when these proteins are bound to their receptors.

Related Publications:
influence of novel CD4 binding-defective hiv-1 envelope glycoprotein immunogens on neutralizing antibody and t-cell responses in nonhuman primates
J. Virol., Feb 2010; 84: 1683 - 1695.
vaccination with an adenoviral vector that encodes and displays a retroviral antigen induces improved neutralizing antibody and CD4+ t-cell responses and confers enhanced protection
J. Virol., Feb 2010; 84: 1967 - 1976.
defects in il-2r signaling contribute to diminished maintenance of foxp3 expression in CD4+cd25+ regulatory t-cells of type 1 diabetic subjects
Diabetes, Feb 2010; 59: 407 - 415.
cellular immune responses to severe acute respiratory syndrome coronavirus (sars-cov) infection in senescent balb/c mice: CD4+ t cells are important in control of sars-cov infection
J. Virol., Feb 2010; 84: 1289 - 1301.
decrease of initial CD4+ t-cell counts at the time of diagnosis of hiv infection in korea; 1988–2006
Int J STD AIDS, Feb 2010; 21: 120 - 125.




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Antibodies:
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