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Mouse Stem Cell Factor Recombinant

SCF

accession:  P20826
   Size A     10ug   $ 160
   Size B      100ug  $480
   Size C      1mg  $ 3700
Domain  :  Four helix core
Gene  :  Kitlg
Catalog no. :  RKP20826

Source:
Optimized DNA sequence encoding Mouse SCF mature chain was expressed in Insect cells.
 
Molecular weight:

Native Mouse Stem Cell Factor is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 18 kDa.

Recombinant SCF is a disulfide-linked monomer protein consisting of 165 amino acid residue subunits, and due to glycosylation migrates as an approximately 23 kDa protein under non-reducing and reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is <2.0 ng/ml, corresponding to a specific activity of > 5 x 105 units/mg.

 
Protein Sequence:
    10         20         30         40         50         60 
MKKTQTWIIT CIYLQLLLFN PLVKTKEICG NPVTDNVKDI TKLVANLPND YMITLNYVAG 

        70         80         90        100        110        120 
MDVLPSHCWL RDMVIQLSLS LTTLLDKFSN ISEGLSNYSI IDKLGKIVDD LVLCMEENAP 

       130        140        150        160        170        180 
KNIKESPKRP ETRSFTPEEF FSIFNRSIDA FKDFMVASDT SDCVLSSTLG PEKDSRVSVT 

       190        200        210        220        230        240 
KPFMLPPVAA SSLRNDSSSS NRKAAKAPED SGLQWTAMAL PALISLVIGF AFGALYWKKK 

       250        260        270 
QSSLTRAVEN IQINEEDNEI SMLQQKEREF QEV
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Stem cell factor was lyophilized from a 0.2 μm filtered solution in 20mM Tris, 5% trehalose, pH 7.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

Stem Cell Factor

SCF is a stromal cell- derived cytokine synthesized by fibroblasts and other cell types. SCF promotes proliferation and early differentiation of cells at the level of multipotential stem cells. It has been suggested that SCF is essential for optimal production of various hematopoietic lineages, mainly because of its ability to prevent apoptosis when it co- stimulates with other cytokines. The receptor for SCF, designated SCFR(CD117), is the oncogene designated as KIT. The biological activities of SCF are synergised considerably by colony stimulating factors GM-CSF and G-CSF, and also by IL7, Epo and some other growth and differentiation factors. In combination with IL7 SCF stimulates the proliferation of pre-B-cells. SCF is also a potent chemoattractant for cells (see also: Chemotaxis), for example, mast cells, expressing the kit receptor. One response to SCF in these cells is a characteristic rearrangement of the actin filaments of the cytoskeleton.

Related Publications:
cardiomyocyte-specific overexpression of human Stem Cell Factor improves cardiac function and survival after myocardial infarction in mice
Circulation, Sep 2009; 120: 1065 - 1074.
Stem Cell Factor and interleukin-2/15 combine to enhance mapk-mediated proliferation of human natural killer cells
Blood, Mar 2009; 113: 2706 - 2714.
human Stem Cell Factor (scf) is a heparin-binding cytokine
J. Biochem., Mar 2009; 145: 275 - 278.
role of Stem Cell Factor and bone marrow-derived fibroblasts in airway remodeling
Am. J. Pathol., Feb 2009; 174: 390 - 400.
Stem Cell Factor signaling collaborates in the development of fip1l1/pdgfr induced chronic eosinophilic leukemia in a murine model
Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 470.




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