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Mouse Interleukin-4 Recombinant


accession:  P07750
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 3500
Domain  :  4_helix_cytokine_core
Gene  :  IL4
Catalog no. :  RKP07750

Optimized DNA sequence encoding Mouse Interleukin-4 mature chain was expressed in Escherichia Coli.

Molecular weight:

Native Mouse Interleukin-4, generated by the proteolytic removal of the signal peptide
and propeptide,the molecule has a calculated molecular mass of approximately 14 kDa.

Recombinant Interleukin-4 is a disulfide-linked monomer protein consisting of 121 amino acid residue subunits,  migrates as an approximately 14 kDa protein under non-reducing and reducing conditions in SDS-PAGE.

>96%, as determined by SDS-PAGE and HPLC

Biological Activity:
The ED(50) was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 0.4 ng/ml, corresponding to a specific activity of ≥ 2x 106 units/mg.

Protein Sequence:
        10         20         30         40         50         60 

        70         80         90        100        110        120 

       130        140 
(*)Complete precursor sequence shown, expressed chain highlighted
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

Interleukin-4 was lyophilized from a 0.2 μm filtered solution in PBS, pH 7.

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.



IL4 is produced mainly by a subpopulation of activated T-cells (Th2) which are the biologically most active helper cells for B-cells and which also secrete IL5 and IL6. Another subpopulation (Th1) also produces IL4 albeit to a lesser extent. Non-T/Non-B-cells of the lineage of mast cells also produce IL4. The extracellular domain of the IL4 receptor is related to the receptors for Epo, IL6, and the beta chain of the IL2 receptor. It has been given the name CD124. Two types of IL4 receptor (IL4R) exist: the type 1 receptor is a heterodimer consisting of CD132 and IL4R-alpha. The type 2 receptor is a heterodimer consisting of IL4R-alpha and IL13R-alpha-1. The biological activities of IL4 are species-specific; mouse IL4 is inactive on human cells and human IL4 is inactive on murine cells. IL4 promotes the proliferation and differentiation of activated B-cells, the expression of MHC class 2 antigens, and of low affinity IgE receptors in resting B-cells. IL4 is probably an autocrine growth modulator for Hodgkins lymphomas.

Related Publications:
complex regulation of tartrate-resistant acid phosphatase (trap) expression by interleukin 4(il-4): il-4 indirectly suppresses receptor activator of nf -kappab ligand (rankl)- mediated trap expression but modestly induces its expression directly
J. Biol. Chem., Sep 2009; 10.1074/jbc.M109.001016.
distinct structural requirements for Interleukin-4 (il-4) and il-13 binding to the shared il-13 receptor facilitate cellular tuning of cytokine responsiveness
J. Biol. Chem., Sep 2009; 284: 24289 - 24296.
favorable impact of the Interleukin-4 receptor allelic variant i75 on the survival of diffuse large b-cell lymphoma patients demonstrated in a large prospective clinical trial
Ann. Onc., Sep 2009; 20: 1548 - 1554.
human pharmacokinetics/pharmacodynamics of an Interleukin-4 and interleukin-13 dual antagonist in asthma
J. Clin. Pharmacol., Sep 2009; 49: 1025 - 1036.
role of periostin and Interleukin-4 in recurrence of pterygia
Invest. Ophthalmol. Vis. Sci., Aug 2009; 10.1167/iovs.09-4022.

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