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Mouse IL-18 Recombinant

IL-18

accession:  P70380
   Size A     5ug   $ 70
   Size B      25ug  $160
   Size C      1mg  $ 4700
Domain  :  IL1
Gene  :  IL18
Catalog no. :  RKP70380
Source:
Optimized DNA sequence encoding mouse Interleukin-18 mature chain was expressed in  E.Coli.

 
Molecular weight:

Native mouse IL-18, generated by the proteolytic removal of the signal peptide
and propeptide, and has a calculated molecular mass of approximately 18 kDa.

Recombinant mouse IL-18  is a disulfide-linked monomer protein consisting of 158 amino acid residue subunits, and migrates as an approximately 18 kDa protein under  reducing conditions in SDS-PAGE.


 
Purity:
>90%, as determined by SDS-PAGE and HPLC

 
Protein Sequence:
        10         20         30         40         50         60 
MAAMSEDSCV NFKEMMFIDN TLYFIPEENG DLESDNFGRL HCTTAVIRNI NDQVLFVDKR 

        70         80         90        100        110        120 
QPVFEDMTDI DQSASEPQTR LIIYMYKDSE VRGLAVTLSV KDSKMSTLSC KNKIISFEEM 

       130        140        150        160        170        180 
DPPENIDDIQ SDLIFFQKRV PGHNKMEFES SLYEGHFLAC QKEDDAFKLI LKKKDENGDK 

       190 
SVMFTLTNLH QS 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant mouse Interleukin-18 was is presented as  lyophilized 0.2 μm filtered solution in PBS pH 7.4.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

IL-18

IL18 is synthesized as a biologically inactive precursor protein that has limited biological activity. The proform of IL18 is processed by one of the caspases, Caspase-1, to generate the 18 kDa bioactive molecule. IL-18 is produced during the acute immune response by macrophages and immature dendritic cells. Interleukin-18 is expressed by a variety of immune and non-immune cells, including monocytes and macrophages. IL18 is one of the pro- inflammatory cytokines. The activities of IL18 appear to be species specific. An important function of IL18 is the regulation of functionally distinct subsets of T-helper cells required for cell mediated immune responses (Nakanishi et al, 2001). IL18 functions as a growth and differentiation factor for Th1 cells. IL18 up-regulates FAS ligand mediated cytotoxic activitiy of murine natural killer cells. IL-18 is part of a complex regulatory circuit involved in causing cell death by apoptosis.

Related Publications:
IL-18 binding protein-expressing mesenchymal stem cells improve myocardial protection after ischemia or infarction
PNAS, Sep 2009; 10.1073/pnas.0908924106.
IL-18 and urinary ngal predict dialysis and graft recovery after kidney transplantation
J. Am. Soc. Nephrol., Sep 2009; 10.1681/ASN.2009030264.
a functional polymorphism in IL-18 is associated with severity of bronchial asthma
Am. J. Respir. Crit. Care Med., Sep 2009; 10.1164/rccm.200905-0652OC.
il-12p40 and IL-18 play pivotal roles in orchestrating the cell-mediated immune response to a poxvirus infection
J. Immunol., Sep 2009; 183: 3324 - 3331.
neutrophil chemokines and their role in IL-18-mediated increase in neutrophil o2– production and intestinal edema following alcohol intoxication and burn injury
Am J Physiol Gastrointest Liver Physiol, Aug 2009; 297: G340 - G347.




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