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Mouse Insulin Like Growth Factor-1 Recombinant

IGF-I

accession:  P05017
   Size A     10ug   $ 70
   Size B      50ug  $160
   Size C      1mg  $ 1350
Domain  :  Insulin
Gene  :  IGF1
Catalog no. :  RKP05017
Source:
Optimized DNA sequence encoding mouse Insulin Like Growth Factor-1 mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Native Mouse IGF-1, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 8 kDa.


Recombinant IGF1 is a disulfide-linked homodimeric protein consisting of 71 amino acid residue subunits, and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells was found to be in the range of 20.0-40.0 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MGKISSLPTQ LFKICLCDFL KIKIHIMSSS HLFYLALCLL TFTSSTTAGP ETLCGAELVD 

        70         80         90        100        110        120 
ALQFVCGPRG FYFNKPTGYG SSIRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPTKAARS 

       130        140        150 
IRAQRHTDMP KTQKEVHLKN TSRGSAGNKT YRM 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant mouse IGF-1 was lyophilized from a 0.2 μm filtered solution in 2.5% glycine, 0.5% sucrose, 0.01% Tween80, 5 mM Glutamic acid, pH 4.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

IGF-I

Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-I is synthesized as two precursor isoforms with N- and alternate C- terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I is identical between isoforms and is generated by proteolytic removal of the N- and C- terminal regions. Mature human IGF-I shares 94% and 96% aa sequence identity with mouse and rat IGF-I, respectively (2), and exhibits cross-species activity. It shares 64% aa sequence identity with mature human IGF-II. Circulating IGF-I is produced by hepatocytes, while local IGF-I is produced by many other tissues in which it has paracrine effects.

Related Publications:
statement by the growth hormone research society on the gh/IGF-I axis in extending health span
J Gerontol A Biol Sci Med Sci, Oct 2009; 64A: 1039 - 1044.
partial primary deficiency of insulin-like growth factor (igf)-i activity associated with igf1 mutation demonstrates its critical role in growth and brain development
J. Clin. Endocrinol. Metab., Sep 2009; 10.1210/jc.2009-0452.
myostatin inhibits IGF-I induced myotube hypertrophy through akt
Am J Physiol Cell Physiol, Sep 2009; 10.1152/ajpcell.00043.2009.
the insulin-like growth factor (igf)-i e-peptides modulate cell entry of the mature IGF-I protein
Mol. Biol. Cell, Sep 2009; 20: 3810 - 3817.
elevated levels of insulin-like growth factor (igf)-i in serum rescue the severe growth retardation of IGF-I null mice
Endocrinology, Sep 2009; 150: 4395 - 4403.




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Antibodies:
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