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Human Growth Hormone Recombinant 192AA

GH1

accession:  P01241
   Size A     100ug   $ 70
   Size B      500ug  $160
   Size C      1mg  $ 300
Domain  :  four helix cytokine core
Gene  :  GH1
Catalog no. :  RKP01241
Source:
Optimized DNA sequence encoding Human Growth Hormone mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Native human Growth Hormone is generated by the proteolytic removal of the signal peptide and propeptide,  the molecule has a calculated molecular mass of approximately 22 kDa.

Recombinant  GH is a disulfide-linked monomeric protein consisting of 192 amino acid residue subunits, and migrates as an approximately 22 kDa protein under non-reducing and reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent  cell proliferation assay using the rat lymphoma, Nb2-11, and was found to be in the range of 0.1 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MATGSRTSLL LAFGLLCLPW LQEGSAFPTI PLSRLFDNAM LRAHRLHQLA FDTYQEFEEA 

        70         80         90        100        110        120 
YIPKEQKYSF LQNPQTSLCF SESIPTPSNR EETQQKSNLE LLRISLLLIQ SWLEPVQFLR 

       130        140        150        160        170        180 
SVFANSLVYG ASDSNVYDLL KDLEEGIQTL MGRLEDGSPR TGQIFKQTYS KFDTNSHNDD 

       190        200        210 
ALLKNYGLLY CFRKDMDKVE TFLRIVQCRS VEGSCGF 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Growth Hormone was lyophilized from a 0.2 μm filtered 0.005mM NaHCO3 solution pH 10.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

Growth Hormone

The physiological activity for which growth hormone is best known is the promotion of growth of bone, cartilage, and soft tissues. Detectable levels of growth hormone are found throughout the remainder of adulthood, suggesting other functions in addition to promotion of growth. Growth hormone may be important for the maintenance of lean body mass; most growth-promoting effects of growth hormone are mediated by IGF-1 the synthesis of which is regulated by growth hormone. The biological activities of growth hormone are mediated by receptors belonging to one of the Cytokine receptor families. Growth hormone has been shown to be produced by T- cells, B-cells, and macrophages. In human lymphocytes growth hormone appears to up-regulate its own expression. Growth hormone appears to act as an enhancer of immune responses and is produced in considerable amounts by T- helper cells.

Related Publications:
Growth Hormone secretagogues and Growth Hormone releasing peptides act as orthosteric super-agonists but not allosteric regulators for activation of the g protein go1 by the ghrelin receptor
Mol. Pharmacol., Oct 2009; 76: 802 - 811.
statement by the Growth Hormone research society on the gh/igf-i axis in extending health span
J Gerontol A Biol Sci Med Sci, Oct 2009; 64A: 1039 - 1044.
the p561t polymorphism of the Growth Hormone receptor gene has an inhibitory effect on mandibular growth in young children
Eur J Orthod, Oct 2009; 31: 536 - 541.
central ghrelin regulates peripheral lipid metabolism in a Growth Hormone-independent fashion
Endocrinology, Oct 2009; 150: 4562 - 4574.
effects of syndyphalin-33 on feed intake and circulating measures of Growth Hormone, cortisol, and immune cell populations in the recently weaned pig
J Anim Sci, Oct 2009; 87: 3218 - 3225.




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