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Human Erythropoietin-alpha Recombinant

EPO

accession:  P01588
   Size A     5ug   $ 70
   Size B      50ug  $160
   Size C      1mg  $ 1450
Domain  :  4 helix cytokine core
Gene  :  EPO
Catalog no. :  RKP01588
Source:
Optimized DNA sequence encoding Human EPO mature chain was expressed in CHO cells.

 
Molecular weight:

Native human Erythropoietin-alpha is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 20 kDa.

Recombinant EPO-a  is a glycosylated disulfide-linked homodimeric protein consisting of  166 amino acid residue subunits, and migrates as an approximately 35 kDa protein under  reducing conditions in SDS-PAGE.


 
Purity:
>98%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
Activity was determined by the dose-dependent proliferation assay using a
factor-dependent human erythroleukemic cell line TF-1 and was found to be 3.8x105 IU/mg.

 
Protein Sequence:
        10         20         30         40         50         60 
MGVHECPAWL WLLLSLLSLP LGLPVLGAPP RLICDSRVLE RYLLEAKEAE NITTGCAEHC 

        70         80         90        100        110        120 
SLNENITVPD TKVNFYAWKR MEVGQQAVEV WQGLALLSEA VLRGQALLVN SSQPWEPLQL 

       130        140        150        160        170        180 
HVDKAVSGLR SLTTLLRALG AQKEAISPPD AASAAPLRTI TADTFRKLFR VYSNFLRGKL 

       190 
KLYTGEACRT GDR 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Erythropoietin alpha  was lyophilized from a 0.2 μm filtered PBS solution  pH 7.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

EPO

EPO is predominantly synthesized and secreted by tubular and juxtatubular capillary endothelial and interstitial cells of the kidney. Approximately 10-15 % of the total amount of Epo comes from extrarenal sources and is predominantly produced by hepatocytes and Kupffer cells of the liver. Approximately 40 % of the molecular mass of Epo is due to its glycosylation. Glycosylation is an important factor determining the pharmacokinetic behavior of Epo in vivo. Non- glycosylated Epo has an extremely short biological half life. It still binds to its receptor and may even have a higher specific activity in vitro.

Related Publications:
a jak2 interdomain linker relays EPO receptor engagement signals to kinase activation
J. Biol. Chem., Sep 2009; 284: 26988 - 26998.
erk- and akt-dependent neuroprotection by erythropoietin (EPO) against glyoxal-ages via modulation of bcl-xl, bax and bad
Invest. Ophthalmol. Vis. Sci., Jul 2009; 10.1167/iovs.09-3544.
the characteristics of technical character and the ongoing saga in the EPO and the english courts
Journal of Intellectual Property Law Practice, Jul 2009; 4: 506 - 514.
comparison of EPOetin alfa (EPO) and darbEPOetin alfa (darb) utilization in cancer chemotherapy patients treated in a hospital outpatient setting
ASCO Meeting Abstracts, May 2009; 27: e17524.
phase ii study of sagopilone (zk-EPO) in patients with recurrent metastatic breast cancer (mbc)
ASCO Meeting Abstracts, May 2009; 27: 1083.




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