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Monoclonal mouse anti human VEGF

VEGF

accession:  P15692
   Size A       $
   Size B      500ug  $400
   Size C      1mg  $ 600
Clone  :  SB767
Isotype  :  IgG1
Catalog no. :  RMA15692
Applications:
ELISA:This antibody can be used at 4 ug/mL with the appropriate secondary reagents to detect human VEGF. The detection limit for recombinant human VEGF is approximately 0.2ng/well.
 
Source:
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant E. coli-derived Human VEGF. 
 
Species reactivity:
Human
 
Purification:

The IgG fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.

 
Presentation:
Lyophilized from sterile filtered solution at a concentration of 1mg/ml in PBS pH 7.4.
 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
 
Storage:
The lyophilized antibody is stable for at least 1 year  from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:

This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

VEGF

VEGF is a homodimeric heavily glycosylated protein.The human factor occurs in several molecular variants of 121, 162, 145, 148, 165, 183, 189 , 206 amino acids, arising by alternative splicing of the mRNA.The splice forms of VEGF differ in biological properties such as the receptor types, which they recognize and their interaction with heparan sulfate proteoglycans. The 165 amino acid form of the factor is the most common form in most tissues. Kaposi sarcomas express VEGF121 and VEGF165. VEGF121 and VEGF165 are soluble secreted forms of the factor while VEGF189 and VEGF206 are mostly bound to heparin-containing proteoglycans in the cell surface or in the basement membrane. A high-affinity glycoprotein receptor of 170-235 kDa is expressed on vascular endothelial cells. The interaction of VEGF with heparin-like molecules of the extracellular matrix is required for efficient receptor binding. Protamine sulfate and suramin are capable of replacing the receptor-bound factor. The high-affinity receptor for VEGF, now known as VEGFR1, has been identified as the gene product of the FLT-1. Another receptor for VEGF, now known as VEGFR2, is KDR, also known as FLK-1. A factor that competes with the 165 amino acid form of VEGF for receptor binding is PLGF. A third receptor type, VEGFR3 is known also as FLT- 4. An isoform-specific receptor for VEGF165 has been identified as human Neuropilin-1.

Related Publications:
decreased nephrin and glepp-1, but increased VEGF, flt-1, and nitrotyrosine, expressions in kidney tissue sections from women with preeclampsia
Reproductive Sciences, Oct 2009; 16: 970 - 979.
significantly higher levels of vascular endothelial growth factor (VEGF) and shorter survival times for patients with primary operable triple-negative breast cancer
Ann. Onc., Oct 2009; 20: 1639 - 1646.
comparative analysis of metastasis variants derived from human prostate carcinoma cells: roles in intravasation of VEGF-mediated angiogenesis and upa-mediated invasion
Am. J. Pathol., Oct 2009; 175: 1638 - 1652.
autocrine loop between vascular endothelial growth factor (VEGF)-c and VEGF receptor-3 positively regulates tumor-associated lymphangiogenesis in oral squamoid cancer cells
Am. J. Pathol., Oct 2009; 175: 1709 - 1721.
polyneuropathy, organomegaly, endocrinopathy, monoclonal protein, skin changes (poems) syndrome refractory to complete serum vascular endothelial growth factor (VEGF) blockade: insights from sequential VEGF monitoring
NDT Plus, Oct 2009; 2: 357 - 359.




by class:
Cytokines
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by species:
Human
mouse
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Antibodies:
Purified Polyclonal
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