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Monoclonal Mouse Anti Human Interleukin-6

IL-6

accession:  RAP05231
   Size A       $
   Size B      500ug  $400
   Size C      1mg  $ 600
Clone  :  SB9H6
Isotype  :  IgG1
Catalog no. :  RMA05231
Applications:
  • Direct ELISA: This antibody can be used at 0.5-1μg/mLwith the appropriate secondary reagents to detect human Interleukin-6. The detection limit for human IL-6 is approximately 2ng/well.
  • Western blot: This monoclonal antibody has been tested in WB analysis of human IL-6 . A suitable range of concentrations of this antibody for WB detection is 0.5-2 µg/ml.
 
Source:
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant E. coli-derived Human IL-6. 
 
Species reactivity:
Human IL-6
 
Purification:

The IgG fraction of the tissue culture supernatant was purified by Protein A affinity chromatography.

 
Presentation:
Prepared from sterile filtered solution at a concentration of 1mg/ml in PBS with 5% trehalose.
 
Storage:
This antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:

This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. 

 

IL-6

IL6 is produced by many different cell types. The main sources in vivo are stimulated monocytes, fibroblasts, and endothelial cells. Macrophages, T-cells and B-lymphocytes, granulocytes, smooth muscle cells, eosinophils, chondrocytes, osteoblasts, mast cells, glial cells, and keratinocytes also produce IL6 after stimulation. IL6 is a member of a family of cytokines, which also includes LIF, CNTF, Oncostatin M, IL11, and CT- 1. All known members of the IL6 cytokine family induce hepatic expression of acute phase proteins. The IL6 receptor is expressed on T-cells, mitogen-activated B-cells, peripheral monocytes and some macrophage- and B-cell derived tumor cell types. It is not expressed in resting B-cells but in resting T- cells. The IL6 receptor is a strongly glycosylated protein of 80 kDa and a length of 449 amino acids. It has been designated CD126.

Related Publications:
IL-6 protects against hyperoxia-induced mitochondrial damage via bcl-2–induced bak interactions with mitofusions
Am. J. Respir. Cell Mol. Biol., Oct 2009; 41: 385 - 396.
long-term safety and efficacy of tocilizumab, an anti-IL-6 receptor monoclonal antibody, in monotherapy, in patients with rheumatoid arthritis (the stream study): evidence of safety and efficacy in a 5-year extension study
Ann Rheum Dis, Oct 2009; 68: 1580 - 1584.
the second bovine β-galactoside-2,6-sialyltransferase (st6gal ii): genomic organization and stimulation of its in vitro expression by IL-6 in bovine mammary epithelial cells
Glycobiology, Oct 2009; 19: 1082 - 1093.
IL-6 -174g/c promoter polymorphism in acute appendicitis
Lab Med, Oct 2009; 40: 600 - 603.
cell surface-bound il-1 is an upstream regulator of the senescence-associated IL-6/il-8 cytokine network
PNAS, Sep 2009; 10.1073/pnas.0905299106.




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Cytokines
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by species:
Human
mouse
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Antibodies:
Purified Polyclonal
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