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Monoclonal Mouse Anti Human GM-CSF

GM-CSF

accession:  P04141
   Size A       $
   Size B      500ug  $400
   Size C      1mg  $ 600
Clone  : 
Isotype  :  IgG1
Catalog no. :  RMA04141
Applications:
  • Neutralization: Data not available.
  • Western blot: This monoclonal antibody has been tested in WB analysis of human GM-CSF . A suitable range of concentrations of this antibody for WB detection is 0.5-2 µg/ml.
  • IHC: Use at a concentration of 1-2 µg/ml.
 
Source:
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant E. coli-derived Human GM-CSF. 
 
Species reactivity:
Human
 
Purification:

The IgG fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.

 
Presentation:
Lyophilized from sterile filtered solution at a concentration of 1mg/ml in citrate-phosphate buffer pH7.0, with 0.02%Proclin 300 as a preservative.
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
 
Storage:
The lyophilized antibody is stable for at least 1 year  from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:

This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

GM-CSF

GM-CSF is a monomeric protein of 127 amino acids with two glycosylation sites. The protein is synthesized as a precursor of 144 amino acids, which included a hydrophobic secretory signal sequence at the aminoterminal end. The sugar moiety is not required for the full spectrum of biological activities. Non- glycosylated and glycosylated GM-CSF show the same activities in vitro. Fully glycosylated GM-CSF is biologically more active in vivo than the non- glycosylated protein. This protein is secreted together with other factors by T- cells and macrophages following cell activation by antigens or mitogens. Approximately 90% of the secreted colony stimulating activities are due to GM- CSF.

Related Publications:
GM-CSF regulates intimal cell proliferation in nascent atherosclerotic lesions
J. Exp. Med., Sep 2009; 206: 2141 - 2149.
the transcription factors stat5a/b regulate GM-CSF-mediated granulopoiesis
Blood, Sep 2009; 10.1182/blood-2009-04-216390.
GM-CSF and il-4 induce dendritic cell differentiation and disrupt osteoclastogenesis through m-csf receptor shedding by up-regulation of tnf- converting enzyme (tace)
Blood, Sep 2009; 10.1182/blood-2009-04-215020.
biologic activity of irradiated, autologous, GM-CSF-secreting leukemia cell vaccines early after allogeneic stem cell transplantation
PNAS, Sep 2009; 106: 15825 - 15830.
stress response genes are suppressed in mouse preimplantation embryos by granulocyte-macrophage colony-stimulating factor (GM-CSF)
Hum. Reprod., Sep 2009; 10.1093/humrep/dep307.




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Antibodies:
Purified Polyclonal
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